Loading starch gels using filter paper wicks.
نویسندگان
چکیده
منابع مشابه
Facilitated loading of horizontal gels using a capillary comb loader.
Manual loading of samples into horizontal gels, such as the agarose gels commonly used for DNA fragment sizing and quantification, is laborious and prone to errors. Manual-loading times for highthroughput gels can reach 10 min/gel, and human error can result in incorrect identification of samples because of reverse loading or other errors in the loading process. To reduce gel-loading times and ...
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متن کاملRheology and Water Swelling of Carboxymethyl Starch Gels
A series of gels differing in ionic content was prepared by derivatizing starch with varying amounts of carboxymethyl add-on. Some derivatives were lightly crosslinked with epichlorohydrin to reduce soluble material and increase the amount of gel. The gel fraction of each batch was isolated. and viscosity 1]. shear modulus G. and swelling in water and salt solution were then determined. One gel...
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The study examined the influence of chitosan, incorporated into whey protein and wheat starch thermo gels, on the in-vitro hydrolysis of the polysaccharide. Gels were subjected to the following external conditions containing α-amylase at constant incubation temperature of 37 °C: In the first procedure, they were immersed in phosphate buffer (0.05 M) and maintained at pH 6.9 throughout the entir...
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Amylomaltases are 4-alpha-glucanotransferases (EC 2.4.1.25) of glycoside hydrolase family 77 that transfer alpha-1,4-linked glucans to another acceptor, which can be the 4-OH group of an alpha-1,4-linked glucan or glucose. The amylomaltase-encoding gene (PAE1209) from the hyperthermophilic archaeon Pyrobaculum aerophilum IM2 was cloned and expressed in Escherichia coli, and the gene product (Py...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1969
ISSN: 0021-9746
DOI: 10.1136/jcp.22.2.242-b